As predicted, the Karl Rove and Ed Gillespie-linked* American Crossroads group has launched an ad in the Kentucky Senate Race. The clip criticizes Democratic candidate Jack Conway for saying he supports the health care overhaul passed in March and for not joining several other state attorneys general in filing suit against the legislation once it became law.

Response ads can be expected, as we’ve heard about two groups planning ads that target Republican candidate Rand Paul.

*Update: American Crossroads says Rove and Gillespie are not formally associated with American Crossroads or Crossroads GPS, though they are “informal advisors.” A number of outlets, however, have reported on the ties between Rove and the group.

Update 2: Here is Conway’s response, from CN2.